Rabbit monoclonal antibodies are developed directly from isolated B cells of immunized animals without the use of hybridomas. The technology allows the sampling of much larger B cell repertoire for the screening and selection of high quality antibodies to suite customers’ application needs. Antigen-specific B cells are cultured in multi-well plates and supernatant samples screened to identify desired antibodies. The cDNAs encoding the H and L chains are isolated and expressed in a mammalian expression system for batch-to-batch consistency and higher yield. Expression-ready cDNA constructs and complete sequences are provided to customers upon project completion, enabling customers to produce the antibodies in-house and to protect the intellectual property.
Highlights of Excel BioPharm Rabbit Monoclonal Services -